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Very few studies have investigated the effects of GPR55 activation on nociceptive processing in vivo and only one at the level of the joint during acute inflammatory arthritis. The aim of this thesis was to investigate the role of GPR55 in the modulation of joint afferent mechanosensitivity in vivo steroids, and whether this role is altered in an experimental model of OA during established pain.Electrophysiological recordings of joint afferent nociceptors, taken from the saphenous nerve, which innervates the knee joint via the medial articular nerve, were carried out in anaesthetised rats under non pathological conditions (nave rats) and in a model of OA following the development of pain behaviour, 14 days following knee joint injection of monosodium iodoacetate (MIA) and compared to saline control rats. Effects of peripheral administration of the putative endogenous GPR55 agonist L lysophosphatidylinositol (LPI) on the mechanically evoked responses of joint nociceptors were studied in nave rats and in MIA and saline rats.
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