Hydrolysis of the saponins present in these extracts decreased

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steriods Crude saponin extracts, in 80% methanol, from alfalfa seedling tissues were bioassayed against the cowpea seed weevil, Callosobruchus maculatus. Both extracts from shoot and root tissues caused larval mortality and delayed development when incorporated into an artificial diet at levels comparable to those found in alfalfa steroids, but lower levels of root saponin extracts showed probiotic effects, whereas lower levels of shoot saponins were still toxic. Hydrolysis of the saponins present in these extracts decreased their toxicity. steriods

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